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Abstract. The Laurentian Great Lakes significantly influence the climate of the Midwest and Northeast United States due to their vast thermal inertia, moisture source potential, and complex heat and moisture flux dynamics. This study presents a newly developed coupled lake–ice–atmosphere (CLIAv1) modeling system for the Great Lakes by coupling the National Aeronautics and Space Administration (NASA) Unified Weather Research and Forecasting (NU-WRF) regional climate model (RCM) with the three-dimensional (3D) Finite Volume Community Ocean Model (FVCOM) and investigates the impact of coupled dynamics on simulations of the Great Lakes' winter climate. By integrating 3D lake hydrodynamics, CLIAv1 demonstrates superior performance in reproducing observed lake surface temperatures (LSTs), ice cover distribution, and the vertical thermal structure of the Great Lakes compared to the NU-WRF model coupled with the default 1D Lake Ice Snow and Sediment Simulator (LISSS). CLIAv1 also enhances the simulation of over-lake atmospheric conditions, including air temperature, wind speed, and sensible and latent heat fluxes, underscoring the importance of resolving complex lake dynamics for reliable regional Earth system projections. More importantly, the key contribution of this study is the identification of critical physical processes that influence lake thermal structure and ice cover – processes that are missed by 1D lake models but effectively resolved by 3D lake models. Through process-oriented numerical experiments, we identify key 3D hydrodynamic processes – ice transport, heat advection, and shear production in turbulence – that explain the superiority of 3D lake models to 1D lake models, particularly in cold season performance and lake–atmosphere interactions. Critically, all three of these processes are dynamically linked to water currents – spatially and temporally evolving flow fields that are structurally absent in 1D models. This study aims to advance our understanding of the physical mechanisms that underlie the fundamental differences between 3D and 1D lake models in simulating key hydrodynamic processes during the winter season, and it offers generalized insights that are not constrained by specific model configurations. 

Abstract The resolution of fluorescence microscopy images is limited by the physical properties of light. In the last decade, numerous super-resolution microscopy (SRM) approaches have been proposed to deal with such hindrance. Here we present Mean-Shift Super Resolution (MSSR), a new SRM algorithm based on the Mean Shift theory, which extends spatial resolution of single fluorescence images beyond the diffraction limit of light. MSSR works on low and high fluorophore densities, is not limited by the architecture of the optical setup and is applicable to single images as well as temporal series. The theoretical limit of spatial resolution, based on optimized real-world imaging conditions and analysis of temporal image stacks, has been measured to be 40 nm. Furthermore, MSSR has denoising capabilities that outperform other SRM approaches. Along with its wide accessibility, MSSR is a powerful, flexible, and generic tool for multidimensional and live cell imaging applications.